Biodegradation Modeling of Nitrophenolic Pollutant in a Slurry Bubble Reactor

Document Type : Original Paper

Authors

1 School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran

2 Chemical Engineering Department, Amirkabir University of Technology, Tehran, Iran

Abstract

Biodegradation kinetics of 4-nitrophenol (PNP) in aqueous solution by a gram negative soil bacterium, Ralstoniaeutropha was firstly studied in a small scale batch reactor. The degradation of PNP was evaluated at initial PNP concentrations ranging from 3 mg/L to 14 mg/L. The rate of PNP consumption by the bacterium culture was modeled using Monod and Contois kinetics in batch condition. PNP degradation by adapted R. eutropha was well fitted to the Monod equation for the pollutant with initial concentration of 3-8 mg/L, whereas for PNP with initial concentration of 14 mg/L the experimental data were better fitted to the Contois kinetic model. The process of degradation of PNP was scaled up in a slurry bubble reactor with 250 ml working volume in presence of 0.75 L/min air flow rate. The derived kinetic model was validated by comparison the outlet time dependent experimental data of PNP concentration with the model in the slurry bioreactor and the results showed good agreement between experiments and the model.

Keywords

References

[1] Chaudhari, R. V. and Ramachandran, P. A. (1983). "Three-phase catalytic reactors." AlChE J., Vol. 26, pp.177-201.
[2] Tomei, M.C., Annesini, M.C. and Bussoletti, S. (2004). "4-Nitrophenol biodegradation in a sequencing batch reactor: kinetic study and effect of filling time." Water Res., Vol. 38, pp.375–384.
[3] Tomei, M.C. and Annesini, M.C. (2005). "4-Nitrophenol biodegradation in a sequencing batch reactor operating with aerobic–anoxic cycles." Environ Sci Technol., Vol. 39, pp. 5059–5065.
[4] Yi, S., Zhuang W.Q., Wu, B., Stay, T.L. and Tay, J. H. (2006). "Biodegradation of pnitrophenol by aerobic granules in a sequencing batch reactor."Environ. Sci. Technol., 40, pp. 2396–2401.
[5] Martin-Hernandez, M., Carrera, J., Perez, J. and Suarez-Ojeda, M.E. (2009). "Enrichment of a K-strategist microbial population able to biodegrade p-nitrophenol in a sequencing batch reactor." Water Res., Vol. 43, pp. 3871–3883.
[6] Salehi, Z., Sohrabi, M., Vahabzadeh, F., Fatemi, S. and Kawase, Y. (2010). "Modeling of p-nitrophenol biodegradation by Ralstoniaeutropha via application of the substrate inhibition concept." J Hazard. Mater., Vol. 177, pp. 582–585.
[7] She, Z., GaoJin, M., C., Chen, Y. and Yu, J. (2005). 'Toxicity and biodegradation of 2,4-dinitrophenol and 3- nitrophenol in anaerobic systems." Process Biochem., Vol. 40, pp. 3017–24.
[8] Qiu, X., Zhong, Q., Li, M., Bai, W. and Li, B. (2007). "Biodegradation of p-nitrophenol by methyl parathion degrading Ochrobactrum sp. B2." Int Biodeterior. Biodegrad., Vol. 59, pp. 297–301.
[9] Muller, R. H. and Babel, W. (1996). "Growth rate-dependent expression of phenol-assimilation pathways in Alcaligeneseutrophus JMP 134 – the influence of formate as an auxiliary energy source on phenol conversion characteristics."Appl. Microbiol. Biotechnol., Vol. 46, pp.156-162.
[10] Salehi, Z., Yoshikawa, H., Mineta, R. and Kawase, Y. (2011). "Aerobic biodegradation of p-nitrophenol by acclimated waste activated sludge in a slurry bubble column." Process. Biochem., Vol. 46, pp. 284–289.
[11] Qiu, X., Wu, P., Zhang, H., Li, M. and Yan, Z. (2009). "Isolation and characterization of Arthrobacter sp. HY2 capable of degrading a high concentration of p-nitrophenol." BioresourTechnol., Vol. 100, pp. 5243– 5248.
[12] Gemini, V.L., Gallego, A., de Oliveira, V.M., Gomez, C.E., Manfio, G.P., SKorol. E.(2005)."Biodegradation and detoxification of p-nitrophenol by Rhodococcuswratislaviensis." Int Biodeterior. Biodegrad., Vol. 55, pp.103–108.
[13] Rezouga, F., Hamdi, M. and Sperandio, M. (2009)."Variability of kinetic parameters due to biomass acclimation: case of para-nitrophenol biodegradation." Bioresour. Technol., Vol. 100, pp. 5021–5029.
[14] Muller, S., Bley, T. and Babel, W. (1999). "Adaptive responses of Ralstoniaeutropha to feast and famine conditions analysed by flow cytometry." J. Biotechnol., Vol. 75, pp.81–97.
[15] Hill, A., Milne, B. J. and Nawrocki, P. A. (1996). "Cometabolic degradation of 4-chlorophenol by Alcaligeneseutrophus." Appl. Microbiol. Biotechnol., Vol. 46, pp.163-168.
[16] Craig Criddle, S. (1993). "The kinetics of cometabolism."Biotechnol. Bioeng., Vol. 41, pp. 1048–1056.
[17] Schmidt, S. K., Simkins, S. and Alexander, M. (1985)."Models for the Kinetics of Biodegradation of Organic Compounds Not Supporting Growth." Appl. Env. Mic., pp. 323-331.
Journal of Chemical and Petroleum Engineering
Volume 48, Issue 2 - Serial Number 2
December 2014
Pages 117-124

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History

  • Receive Date: 04 March 2014
  • Accept Date: 09 September 2014

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