Investigation of Operating Parameters on Ultrasound-Assisted Extraction of Anethole in Fennel Essential Oil

Document Type : Research Paper

Authors

1 Surface Phenomenon and Liquid-Liquid Extraction Research Lab, School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran

2 Fouman Faculty of Engineering, College of Engineering, University of Tehran, Tehran, Iran

Abstract

In this paper, the operational impact of three parameters including power of ultrasonic apparatus, size of fennel seeds and experiment time on the extraction yield of Anethole, which is the main considerable component in fennel essential oil and its concentration have been studied through Ultrasound-Assisted Extraction. The ultrasonic extraction of oil from fennel seeds using a solution of 70% water-ethanol was studied at different particle sizes, different ultrasonic powers and three different levels of time. The most effective parameter was particle size, while the experiment time had the least impact on both the efficiency and Anethole concentration as well. As a result, compared to Soxhlet method, the ultrasonic-assisted extraction was more efficient. In this experiment, eighteen constituents were identified for fennel seeds using GC–MS. The major components were Anethole (78.12%), Fenchone (8.81%), Limonene (4.39%), and Estragole (4.52%). Furthermore, the analysis of two quadratic models using the Box-Behnken design (BBD) indicated that the quadratic polynomial model can be applied for estimating the Anethole extraction yield as well as Anethole concentration.

Keywords

References

  1. Bruni R, Muzzoli M, Ballero M, Loi MC, Fantin G, Poli F, et al. Tocopherols, fatty acids and sterols in seeds of four Sardinian wild Euphorbia species. Fitoterapia. 2004;75(1):50-61.
  2. Cai F, Li Y, Zhang M, Zhang H, Wang Y, Hu P. Combination of integrated expanded bed adsorption chromatography and countercurrent chromatography for the direct extraction and purification of pseudohypericin and hypericin from St. John's wort (Hypericum perforatum L.). Journal of separation science. 2015;38(15):2588-96.
  3. Mohamad RH, El-Bastawesy AM, Abdel-Monem MG, Noor AM, Al-Mehdar HAR, Sharawy SM, et al. Antioxidant and anticarcinogenic effects of methanolic extract and volatile oil of fennel seeds (Foeniculum vulgare). Journal of medicinal food. 2011;14(9):986-1001.
  4. Moubarik A, El-Belghiti K, Vorobiev E. Kinetic model of solute aqueous extraction from Fennel (Foeniculum vulgare) treated by pulsed electric field, electrical discharges and ultrasonic irradiations. Food and bioproducts processing. 2011;89(4):356-61.
  5. Anwar F, Ali M, Hussain AI, Shahid M. Antioxidant and antimicrobial activities of essential oil and extracts of fennel (Foeniculum vulgare Mill.) seeds from Pakistan. Flavour and Fragrance Journal. 2009;24(4):170-6.
  6. Friedman M. Chemistry and multibeneficial bioactivities of carvacrol (4-isopropyl-2-methylphenol), a component of essential oils produced by aromatic plants and spices. Journal of agricultural and food chemistry. 2014;62(31):7652-70.
  7. Oktay M, Gülçin İ, Küfrevioğlu Öİ. Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. LWT-Food Science and Technology. 2003;36(2):263-71.
  8. Albu S, Joyce E, Paniwnyk L, Lorimer JP, Mason TJ. Potential for the use of ultrasound in the extraction of antioxidants from Rosmarinus officinalis for the food and pharmaceutical industry. Ultrasonics sonochemistry. 2004;11(3-4):261-5.
  9. Mokhtari L, Ghoreishi SM. Supercritical carbon dioxide extraction of trans-anethole from Foeniculum vulgare (fennel) seeds: Optimization of operating conditions through response surface methodology and genetic algorithm. Journal of CO2 Utilization. 2019;30:1-10.
  10. Tognolini M, Ballabeni V, Bertoni S, Bruni R, Impicciatore M, Barocelli E. Protective effect of Foeniculum vulgare essential oil and anethole in an experimental model of thrombosis. Pharmacological research. 2007;56(3):254-60.
  11. Damayanti A, Setyawan E. Essential oil extraction of fennel seed (Foeniculum vulgare) using steam distillation. International Journal of Science and Engineering. 2012;3(2):12-4.
  12. Pham HNT, Bowyer MC, van Altena IA, Scarlett CJ. Influence of solvents and novel extraction methods on bioactive compounds and antioxidant capacity of Phyllanthus amarus. Chemical Papers. 2016;70(5):556-66.
  13. Moo-Huchin VM, Canto-Pinto JC, Cuevas-Glory LF, Sauri-Duch E, Pérez-Pacheco E, Betancur-Ancona D. Effect of extraction solvent on the phenolic compounds content and antioxidant activity of Ramon nut (Brosimum alicastrum). Chemical Papers. 2019:1-11.
  14. Lopes AP, Petenuci ME, Galuch MB, Schneider VVA, Canesin EA, Visentainer JV. Evaluation of effect of different solvent mixtures on the phenolic compound extraction and antioxidant capacity of bitter melon (Momordica charantia). Chemical Papers. 2018;72(11):2945-53.
  15. Abu-Arabi MK, Allawzi MA, Al-Zoubi HS, Tamimi A. Extraction of jojoba oil by pressing and leaching. Chemical Engineering Journal. 2000;76(1):61-5.
  16. Damjanović B, Lepojević Ž, Živković V, Tolić A. Extraction of fennel (Foeniculum vulgare Mill.) seeds with supercritical CO2: Comparison with hydrodistillation. Food Chemistry. 2005;92(1):143-9.
  17. Lucchesi ME, Chemat F, Smadja J. Solvent-free microwave extraction of essential oil from aromatic herbs: comparison with conventional hydro-distillation. Journal of Chromatography A. 2004;1043(2):323-7.
  18. Toma M, Vinatoru M, Paniwnyk L, Mason TJ. Investigation of the effects of ultrasound on vegetal tissues during solvent extraction. Ultrasonics sonochemistry. 2001;8(2):137-42.
  19. Gupta A, Naraniwal M, Kothari V. Modern extraction methods for preparation of bioactive plant extracts. International journal of applied and natural sciences. 2012;1(1):8-26.
  20. Kimbaris AC, Siatis NG, Daferera DJ, Tarantilis PA, Pappas CS, Polissiou MG. Comparison of distillation and ultrasound-assisted extraction methods for the isolation of sensitive aroma compounds from garlic (Allium sativum). Ultrasonics sonochemistry. 2006;13(1):54-60.
  21. Lambert RJW, Skandamis PN, Coote PJ, Nychas GJ. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. Journal of applied microbiology. 2001;91(3):453-62.
  22. Chen Y, Zhang D, Gao R, Gao L. Experimental and modeling analysis of flavones extraction from Vaccinium bracteatum Thunb. leaves by ultrasound and microwave assisted simultaneously. Chemical Papers. 2019;73(4):783-90.
  23. Chen ML, Yang DJ, Liu SC. Effects of drying temperature on the flavonoid, phenolic acid and antioxidative capacities of the methanol extract of citrus fruit (Citrus sinensis (L.) Osbeck) peels. International Journal of Food Science & Technology. 2011;46(6):1179-85.
  24. Yang B, Zhao M, Shi J, Yang N, Jiang Y. Effect of ultrasonic treatment on the recovery and DPPH radical scavenging activity of polysaccharides from longan fruit pericarp. Food chemistry. 2008;106(2):685-90.
  25. Liao J, Zheng N, Qu B. An improved ultrasonic-assisted extraction method by optimizing the ultrasonic frequency for enhancing the extraction efficiency of lycopene from tomatoes. Food analytical methods. 2016;9(8):2288-98.
  26. Paniwnyk L, Beaufoy E, Lorimer JP, Mason TJ. The extraction of rutin from flower buds of Sophora japonica. Ultrasonics sonochemistry. 2001;8(3):299-301.
  27. Yolmeh M, Najafi MBH, Farhoosh R. Optimisation of ultrasound-assisted extraction of natural pigment from annatto seeds by response surface methodology (RSM). Food chemistry. 2014;155:319-24.
  28. Xu D-P, Zheng J, Zhou Y, Li Y, Li S, Li H-B. Ultrasound-assisted extraction of natural antioxidants from the flower of Limonium sinuatum: Optimization and comparison with conventional methods. Food chemistry. 2017;217:552-9.
  29. Qu Y, Li C, Zhang C, Zeng R, Fu C. Optimization of infrared-assisted extraction of Bletilla striata polysaccharides based on response surface methodology and their antioxidant activities. Carbohydrate polymers. 2016;148:345-53.
  30. Jang S, Lee AY, Lee AR, Choi G, Kim HK. Optimization of ultrasound-assisted extraction of glycyrrhizic acid from licorice using response surface methodology. Integrative medicine research. 2017;6(4):388-94.
  31. Bondy SC. Ethanol toxicity and oxidative stress. 1992.
  32. Ma Y-Q, Chen J-C, Liu D-H, Ye X-Q. Simultaneous extraction of phenolic compounds of citrus peel extracts: effect of ultrasound. Ultrasonics sonochemistry. 2009;16(1):57-62.
  33. Ben-Ali S, Akermi A, Mabrouk M, Ouederni A. Optimization of extraction process and chemical characterization of pomegranate peel extract. Chemical Papers. 2018;72(8):2087-100.
  34. Jiang L, Wang J, Li Y, Wang Z, Liang J, Wang R, et al. Effects of ultrasound on the structure and physical properties of black bean protein isolates. Food Research International. 2014;62:595-601.
  35. Zhou Y, Zheng J, Gan R-Y, Zhou T, Xu D-P, Li H-B. Optimization of ultrasound-assisted extraction of antioxidants from the mung bean coat. Molecules. 2017;22(4):638.
Journal of Chemical and Petroleum Engineering
Volume 55, Issue 2
December 2021
Pages 339-351

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History

  • Receive Date: 05 June 2021
  • Revise Date: 13 August 2021
  • Accept Date: 14 August 2021

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