Optimization of the Sono-Biodiesel in the Attendance of ZnO Nanoparticles, Process Yield Enhancement: Box Behnken Design

Document Type : Research Paper


Department of Chemical Engineering, Faculty of Engineering, University of Guilan, Rasht, Iran


The growing fuel demands and drastic restrictions of politics on greenhouse gases emissions are motivating towards bioenergy research. In this paper, the yield improvement of the produced biodiesel from canola oil using transesterification process, in attendance of ZnO nanocatalyst and ultrasound waves, was investigated. The crystal size, morphology, and particle size of the prepared nanoparticles were recognized applying X-ray diffraction (XRD), scanning electron microscopy (SEM) and transverse electron microscopy (TEM) analyses, respectively. The size of ZnO nanoparticles was 45 nm with a hexagonal-shaped structure. The response surface methodology (RSM) and the Box Behnken design (BBD) were employed to analyze the impact of the independent variables on biodiesel production yield. The reliability of the proposed model was verified by applying the analysis of variance (ANOVA) with the objective of evaluating response. Regarding the yield, satisfactory accordance was obtained between the calculated and prognosticated data from RSM, with R2= 0.9910 and R2 adj= 0.9748. The optimum reaction conditions were acquired at methanol to oil molar ratio of 11.19:1 mol: mol, ultrasound irradiation time of 31.98 min, and nanocatalyst amount of 3.17 wt.%. The optimum value for the sono-biodiesel yield was achieved equal to 90.16%. Moreover, the kinetic study exhibited that the values of activation energy and Arnius frequency factor were achieved 46.23 kJ mol-1 and 5.83× 105 min-1, respectively. Accordingly, this research indicated that ZnO nanoparticles can be utilized as a promising and efficient heterogeneous catalyst for biodiesel production.


  1. Athar M, Zaidi S. A review of the feedstocks, catalysts, and intensification techniques for sustainable biodiesel production. Journal of Environmental Chemical Engineering. 2020;8: 104523.
  2. Foroutan R, Mohammadi R, Razeghi J, Ramavandi B. Biodiesel production from edible oils using algal biochar/CaO/K2CO3 as a heterogeneous and recyclable catalyst. Renewable Energy. 2021;168: 1207-1216.
  3. Maleki B, Ashraf Talesh S. S. Optimization of ZnO incorporation to αFe2O3 nanoparticles as an efficient catalyst for biodiesel production in a sonoreactor: Application on the CI engine. Renewable Energy. 2022; 182: 43-59.
  4. De A, Boxi SS. Application of Cu impregnated TiO2 as a heterogeneous nanocatalyst for the production of biodiesel from palm oil. Fuel. 2020; 256: 117019.
  5. Banerjee, S. Rout, S. Banerjee, A. Atta, D. Das, Fe2O3 nanocatalyst aided transesterification for biodiesel production from lipid-intact wet microalgal biomass: a biorefinery approach, Energy Convers. Management. 2019;195, 844-853.
  6. Rao AVRK, Dudhe P, Chelvam V. Role of oxygen defects in basicity of Se doped ZnO nanocatalyst for enhanced triglyceride transesterification in biodiesel production. Catalysis Communications. 2021; 149:106258.
  7. Suresh T, Sivarajasekar N, Balasubramani K. Enhanced ultrasonic assisted biodiesel production from meat industry waste (pig tallow) using green copper oxide nanocatalyst: Comparison of response surface and neural network modelling, Renewable Energy. 2021;164: 897-907.
  8. Vardast N, Haghighi M, Dehghani S. Sono-dispersion of calcium over Al-MCM-41used as a nanocatalyst for biodiesel production from sunflower oil: influence of ultrasound irradiation and calcium content on catalytic properties and performance. Renewable Energy. 2019; 132: 979–988.
  9. Bai L, Tajikfar A, Tamjidi S, Foroutan R, Esmaeili H. Synthesis of MnFe2O4@graphene oxide catalyst for biodiesel production from waste edible oil. Renewable Energy. 2021;170: 426-437.
  10. Baskar G, Selvakumari AEI, Aiswarya R. Biodiesel production from castor oil using heterogeneous Ni doped ZnO nanocatalyst. Bioresource Technology. 2018; 250: 793- 798.
  11. Dantas J, Leal E, Mapossa AB, Cornejo DR, Costa ACFM. Magnetic nanocatalysts of NiO.5ZnO.5Fe2O4 doped with Cu and performance evaluation in transesterification reaction for biodiesel production. Fuel. 2017;191: 463-471.
  12. Santillán-Urquiza E, Arteaga-Cardona F, Hernandez-Herman E, Pacheco-García MPF. Inulin as a novel biocompatible coating: Evaluation of surface affinities toward CaHPO4, a-Fe2O3, ZnO, CaHPO4@ZnO and a-Fe2O3@ZnO nanoparticles. Journal of Colloid and Interface Science. 2015;460: 339-348.
  13. Jookjantra K, Wongwuttanasatian, T. Optimisation of biodiesel production from refined palm oil with heterogeneous CaO catalyst using pulse ultrasonic waves under a vacuum condition. Energy Conversion and Management. 2017;154: 1-10.
  14. Malani RS, Shinde V, Ayachit S, Goyal A, Moholkar VS. Ultrasound–Assisted Biodiesel Production Using Heterogeneous Base Catalyst and Mixed Non–edible Oils. Ultrasonics Sonochemistry. 2019; 52: 232-243.
  15. Hoseini SS, Najafi G, Ghobadian B, Mamat R, Ebadi MT, Yusaf T. Characterization of biodiesel production (Ultrasonic-assisted) from Evening-primroses (Oenothera lamarckiana) as novel feedstock and its effect on CI engine parameters. Renewable Energy. 2018;130: 50-60.
  16. Selvakumar P, Sivashanmugam P. Ultrasound assisted oleaginous yeast lipid extraction and garbage lipase catalyzed transesterification for enhanced biodiesel production. Energy Conversion and Management. 2019;179: 141-151.
  17. Hoseinia SS, Najafia G, Ghobadiana B, Mamatb R, Ebadia MT, Yusaf T. Ailanthus altissima (tree of heaven) seed oil: Characterisation and optimisation of ultrasonication-assisted biodiesel production. Fuel. 2018; 220: 621- 630.
  18. Maleki B, Ashraf Talesh S.S. Pour point and yield simultaneous improvement of alkyl esters produced by ultrasound-assisted in the presence of αFe2O3/ZnO: RSM approach. Fuel. 2021; 298, 120827.
  19. Elango RK, Sathiasivan K, Muthukumaran C, Thangavelu V, Rajesh M, Tamilarasan K. Transesterification of castor oil for biodiesel production: Process optimizationand characterization. Microchem. 2019;145: 1162–8.
  20. Anwar M, Rasul MG, Ashwath N. Production optimization and quality assessment of papaya (Carica papaya) biodiesel with response surface methodology. Energy Conversion and Management. 2018; 156:103-112.
  21. Mohamed MM, Bayoumy WA, El-Faramawy H, El-Dogdog W, Mohamed AA. A novel α-Fe2O3/AlOOH (γ-Al2O3) nanocatalyst for efficient biodiesel production from waste oil: Kinetic and thermal studies. Renewable Energy. 2020;160: 450-464.
  22. Suryavanshi RD, Mohite SV, Bagade AA, Rajpure KY. Photoelectrocatalytic activity of spray deposited Fe2O3/ZnO photoelectrode for degradation of salicylic acid and methyl orange dye under solar radiation. Materials Science and Engineering: B. 2019 Sep 1;248:114386.
  23. Song X, Li L, Chen X, Xu Q, Song B, Pan Z, Liu Y, Juan F, Xu F, Cao B. Enhanced triethylamine sensing performance of α-Fe2O3 nanoparticle/ZnO nanorod heterostructures. Sensors and Actuators B: Chemical. 2019 Nov 1;298:126917.
  24. Samimi A, Zakeri M, Maleki B, Mohebbi Kalhori D. Experimental and statistical assessments of the mechanical strength reliability of gamma alumina catalyst supports. Particuology. 2015;21: 74-81.
  25. Simsek S, Uslu S. Investigation of the effects of biodiesel/2-ethylhexyl nitrate (EHN) fuel blends on diesel engine performance and emissions by response surface methodology (RSM). Fuel. 2020; 275: 118005.
  26. Singh H, Abhishek Sharma P, Kumar N, Singh Y. Biodiesel yield and properties optimization from Kusum oil by RSM. Fuel. 2021; 291:120218.
  27. Asif S, Ahmad M, Bokhari A, Chuah LF, Klemeš JJ, Akbar MM, Sultana S, Yusup S. Methyl ester synthesis of Pistacia khinjuk seed oil by ultrasonic-assisted cavitation system. Industrial Crops and Products. 2017 Dec 1;108:336-47.
  28. Choudhury HA, Chakma S, Moholkar VS. Mechanistic insight into sonochemical biodiesel synthesis using heterogeneous base catalyst. Ultrasonics sonochemistry. 2014 Jan 1;21(1):169-81.
  29. Kumar G. Ultrasonic-assisted reactive-extraction is a fast and easy method for biodiesel production from Jatropha curcas oilseeds. Ultrasonics sonochemistry. 2017 Jul 1;37:634-9.
  30. Korkut I, Bayramoglu M. Selection of catalyst and reaction conditions for ultrasound assisted biodiesel production from canola oil. Renewable energy. 2018 Feb 1;116:543-51.
  31. Raheem I, Mohiddin MN, Tan YH, Kansedo J, Mubarak NM, Abdullah MO, Ibrahim ML. A review on influence of reactor technologies and kinetic studies for biodiesel application. Journal of Industrial and Engineering Chemistry. 2020 Aug 29.
  32. Ashok A, Ratnaji T, Kennedy LJ, Vijaya JJ, Pragash RG. Magnetically recoverable Mg substituted zinc ferrite nanocatalyst for biodiesel production: Process optimization, kinetic and thermodynamic analysis. Renewable Energy. 2021 Jan 1;163:480-94.